number version or unknown translocations can lead to not the case self-confident calling off CO and you can gene conversion occurrences [thirty six,37]. Since drones from the same nest are the haploid progenies of a diploid queen, it’s efficient in order to de-tect and take off the newest countries that have content number varia-tions because of the discovering the latest hetSNPs in these drones’ sequences (Tables S2 and you can S3 inside the Extra file dos; select tricks for details). 1%, 13.9%, and you will 13.8% of one’s genome, have been understood and discarded off territories I, II, and you will III, respectively (Desk S3 in the A lot more document 2).
In downstream analyses we overlooked such gap with which has web sites unless of course if not indexed
To test the accuracy of your markers one passed the filters, around three drones at random chose of nest I was basically sequenced double individually, plus inde-pendent library framework (Table S1 within the More document dos). Theoretically, an exact (or real) marker was ex-pected getting called in both series from sequencing, be-cause the sequences come from a comparable drone. When a great marker exists in just that round of your sequencing, this marker might possibly be incorrect. By the evaluating these rounds off sequencings, merely 10 from the 671,674 called markers inside the for each drone were seen become disagree-ent due to the mapping errors off checks out, recommending that called indicators are reputable. The fresh new heterozygosity (amount of nucleotide differences for every site) try approxi-mately 0.34%, 0.37%, and you will 0.34% between the two haplo-versions within territories I, II, and III, correspondingly, whenever assessed with these legitimate indicators. (more…)