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Test resource, DNA extraction, and genome sequencing

Posted by RajeevKumar

Test resource, DNA extraction, and genome sequencing

Right here by whole genome sequencing off 55 honey bees and also by design a leading resolution recombination chart in the honey bee, i discovered that crossovers are of this GC articles, nucleotide diversity, and you may gene thickness. We plus affirmed the former idea that genetics expressed within the worker minds enjoys unusually high CO costs. All of our research hold the consider one to diversification out of employee decisions, not resistant means, are a driver of the highest crossing-more than price inside the bees. We discover no facts that crossing-more than speed is followed closely by a premier NCO speed.

Methods and you will product

Five colonies out of honeybees (Apis mellifera ligustica Spin) was amassed from a great bee farm for the Zhenjiang, Asia. For each and every colony consisted of that king, dozens of drones, and you will a huge selection of workers. Bees of about three colonies was indeed selected to possess whole genome sequencing.

The newest DNA of each and every individual is actually extracted having fun with phenol/chloroform/isoamyl liquor strategy. To reduce the risk of bacterial contaminants, new stomachs out of bees were got rid of ahead of DNA extraction. In the 3 ?g from DNA out-of each test were used to own whole genome resequencing just like the left DNA is remaining getting PCR and you will Sanger sequencing. Build of DNA libraries and you can Illumina sequencing were performed on BGI-Shenzhen. From inside the temporary, paired-end sequencing libraries having insert measurements of five-hundred bp have been created for each attempt with respect to the manufacturer’s recommendations. Up coming dos ? 100 bp coordinated-prevent reads was in fact made toward IlluminaHiSEq 2000. The latest queens was in fact sequenced during the approximately 67? exposure normally, drones in the as much as 35? (more…)